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CapeBio™ Technologies

For cDNA synthesis and specialized applications

The CapeMMLV™ Reverse transcriptase is the wild-type Moloney Murine Leukemia Virus (MMLV). It is a 75 kDa recombinant enzyme expressed and purified from a cloned Escherichia coli (E. coli) strain with an enhanced cDNA synthesis performance. This enzyme is an RNA-dependent polymerase that catalyses the synthesis of RNA into cDNA. Suited for synthesizing cDNA from RNA, the enzyme cannot be used directly for PCR targeted gene amplification and must be coupled with Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications.

  • Second strand cDNA synthesis, see protocol for first-strand cDNA synthesis
  • DNA labelling
  • Reverse transcriptase using quantitative PCR
  • Analysis of RNA by primer extension
HomeEnzymes CapeMMLV™ Reverse Transcription Kit
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  • Description
Description

Ordering Info

CapeMMLV™ Reverse Transcription Kit
Contents CapeMMLV™ Reverse transcription, CapeMMLV™ 10X RT Buffer, and Nuclease-free water
Cat. no CME0050 CME0250 CME1000
No. of reactions 50 250 1000

Product Performance

No information available

Product Specifications

Specification
Applications Reverse transcriptase PCR or cDNA synthesis
Real-time or endpoint Both
dNTP's included No
Single or multiplex Both
Hotstart No

Tools & Resources

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  • Quick start protocols View
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  • MSDS View
  • Links to calculation tools or primer design/Tm

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