Standard and specialized ligation experiments

The CapeExpress™ DNA ligation ReadyMix™ is a mastermix designed to limit handling procedures. The enzyme mix is used for catalysis of the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, ligates DNA fragments with either cohesive or blunt termini. The enzyme requires ATP as a cofactor.

• Cloning of restriction enzyme generated fragments
• QuickChange Mutation
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids