Standard and specialized ligation experiments

The Capebio™ T4 DNA Ligation ReadyMix™ is a mastermix designed to limit handling procedures Capebio™ T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, ligates DNA fragments with either cohesive or blunt termini. The enzyme requires ATP as a cofactor.

• Cloning of restriction enzyme generated fragments
• QuickChange Mutation
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Cloning of PCR generated products
• Self-circulation of linear DNA
• AFLP
• Ligation of double-stranded oligonucleotide linkers or adapters to DNA