High-fidelity DNA Polymerases

High-fidelity DNA Polymerases​

APPLICATIONS

  • Routine PCR amplification of DNA fragments
  • Colony PCR
  • Crude sample (lower purity samples)
  • Generation of PCR product for TA cloning.
  • DNA labelling
  • DNA sequencing
  • Short- and Long-range amplifications
  • High-Fidelity PCR applications
  • Can be formulated for qPCR
  • Can be formulated for molecular diagnostics Multiplex qPCR

CapeHi-Fidelity Pfu PCR Kit™

The DNA polymerase is a thermostable, high-fidelity enzyme from the bacterium, Pyrococcus furiosus. The enzyme catalyses 5’→3’ synthesis of DNA, with strong 3’→5’ exonuclease (proofreading) activity. The potential incorporation of base-pair mismatched during polymerization are prevented by its highly efficient proofreading capabilities. It is, therefore, highly used in routine PCR experiments requiring high efficiency. This DNA polymerase has high DNA sequence divergences from other wildtype enzymes, which provides it with superior performance during amplification and proofreading applications.

 Kit Components:

  • CapeHi-Fidelity Pfu DNA Polymerase™ (4 U/µl)
  • CapeSprint PCR Buffer™(10X)
  • dNTPs Mix (10 mM each)
  • MgCl2 Solution
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (Units)
CHP0501 CapeHi-Fidelity Pfu PCR Kit™ 50
CHP01001 CapeHi-Fidelity Pfu PCR Kit™ 100
CHP02501 CapeHi-Fidelity Pfu PCR Kit™ 250
CHP05001 CapeHi-Fidelity Pfu PCR Kit™ 500
CHP010001 CapeHi-Fidelity Pfu PCR Kit™ 1000

CapeHi-Fidelity Pfu PCR ReadyMix Kit™

The product has been formulated for fast and efficient experimental preparation. The CapeHi-Fidelity Pfu PCR ReadyMix™ contains enzyme, buffer, dNTPs, MgCl2 solution at ratios that provide desired experimental results.

Kit Components:

  • CapeHi-Fidelity Pfu PCR ReadyMix™
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (rxns)
CHPM050 CapeHi-Fidelity Pfu PCR ReadyMix Kit™ 50
CHPM0100 CapeHi-Fidelity Pfu PCR ReadyMix Kit™ 100
CHPM0250 CapeHi-Fidelity Pfu PCR ReadyMix Kit™ 250
CHPM0500 CapeHi-Fidelity Pfu PCR ReadyMix Kit™ 500
CHPM01000 CapeHi-Fidelity Pfu PCR ReadyMix Kit™ 1000

CapeHi-Fidelity Pfu DNA Polymerase™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (Units)
CHPE050 CapeHi-Fidelity Pfu DNA Polymerase™ (enzyme only) 50
CHPE0100 CapeHi-Fidelity Pfu DNA Polymerase™ (enzyme only) 100
CHPE0250 CapeHi-Fidelity Pfu DNA Polymerase™ (enzyme only) 250
CHPE0500 CapeHi-Fidelity Pfu DNA Polymerase™ (enzyme only) 500
CHPE01000 CapeHi-Fidelity Pfu DNA Polymerase™ (enzyme only) 1000

Product shipment and recommended storage conditions:

Complete kits and/or individual kit components are shipped in unique CapeBio freezer-safe storage boxes that are placed on dry ice for 2-5 days (international shipments) or on ice packs for 1-2 days (local shipments). Upon arrival of our products to your facility, we advise you to make aliquots & store at -20°C. Once thawed, the Ligation buffer can be stored at 4°C.

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