Ligases

Ligases

APPLICATIONS

  • Cloning of restriction enzyme generated DNA fragments
  • Quick Change or Site-directed mutagenesis
  • Cloning of PCR generated products
  • Amplified fragment length polymorphism (AFLP)
  • Self-circularization of linear DNA
  • Joining of double-stranded oligonucleotide linkers or adaptors to DNA
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids

Express DNA Ligation kit™

The DNA ligase catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. All DNA ligases require ATP as a cofactor. However, Express DNA Ligase™ is unique and has been demonstrated to ligate DNA molecules without using ATP as a co-factor, enabling it to function in wide range of buffer conditions. Moreover, it has shown to be a highly stable enzyme that exhibits activity even after three years of freeze-thawing cycles.

Kit Components:

  • Express DNA Ligase™ (2 U/µl)
  • Express DNA Ligation Buffer™ (5X)
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue NumberProduct Trade NameKit Size (Units)
Exp0501Express DNA Ligation Kit™50
Exp01001Express DNA Ligation Kit™100
Exp02501Express DNA Ligation Kit™250
Exp05001Express DNA Ligation Kit™500
Exp010001Express DNA Ligation Kit™1000

Express DNA Ligase ReadyMix Kit™

The product has been formulated for fast and efficient experimental preparation. The Express DNA Ligation ReadyMix™ contains enzyme and buffer at ratios that provide desired experimental results.

Kit Components:

  • Express DNA Ligation ReadyMix™
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (rxns)
ExpM050 Express DNA Ligation ReadyMix Kit™ 50
ExpM0100 Express DNA Ligation ReadyMix Kit™ 100
ExpM0250 Express DNA Ligation ReadyMix Kit™ 250
ExpM0500 Express DNA Ligation ReadyMix Kit™ 500
ExpM01000 Express DNA Ligation ReadyMix Kit™ 1000

Express DNA Ligase™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (Units)
ExpE050 Express DNA Ligase™ (enzyme only) 50
ExpE0100 Express DNA Ligase™ (enzyme only) 100
ExpE0250 Express DNA Ligase™ (enzyme only) 250
ExpE0500 Express DNA Ligase™ (enzyme only) 500
ExpE01000 Express DNA Ligase™ (enzyme only) 1000

Express Plus DNA Ligation Kit™

The DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. All DNA Ligases require ATP as a cofactor. Express Plus DNA Ligase™ is a proprietary and tested cocktail of both T4 and Express DNA Ligase™, formulated to perform in a wide range of applications.

Kit Components:

  • Express Plus DNA Ligase™ (2 U/µl)
  • Express Plus DNA Ligation Buffer™ (10X)
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (Units)
ExpP0501 Express Plus DNA Ligation Kit™ 50
ExpP01001 Express Plus DNA Ligation Kit™ 100
ExpP02501 Express Plus DNA Ligation Kit™ 250
ExpP05001 Express Plus DNA Ligation Kit™ 500
ExpP010001 Express Plus DNA Ligation Kit™ 1000

Express Plus DNA Ligase ReadyMix Kit™

The product has been formulated for fast and efficient experimental preparation. The Express Plus DNA Ligation ReadyMix™ contains enzyme and buffer at ratios that provide desired experimental results.

 Kit Components:

  • Express Plus DNA Ligation ReadyMix™
  • Extra Pure PCR-grade Water™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (rxns)
ExpPM050 Express Plus DNA Ligation ReadyMix Kit™ 50
ExpPM0100 Express Plus DNA Ligation ReadyMix Kit™ 100
ExpPM0250 Express Plus DNA Ligation ReadyMix Kit™ 250
ExpPM0500 Express Plus DNA Ligation ReadyMix Kit™ 500
ExpPM01000 Express Plus DNA Ligation ReadyMix Kit™ 1000

Express Plus DNA Ligase™

Available Kits:

CapeBio Catalogue Number Product Trade Name Kit Size (Units)
ExpPE050 Express Plus DNA Ligase™ (enzyme only) 50
ExpPE0100 Express Plus DNA Ligase™ (enzyme only) 100
ExpPE0250 Express Plus DNA Ligase™ (enzyme only) 250
ExpPE0500 Express Plus DNA Ligase™ (enzyme only) 500
ExpPE01000 Express Plus DNA Ligase™ (enzyme only) 1000

Testimonials

Product shipment and recommended storage conditions:

Complete kits and/or individual kit components are shipped in unique CapeBio freezer-safe storage boxes that are placed on dry ice for 2-5 days (international shipments) or on ice packs for 1-2 days (local shipments). Upon arrival of our products to your facility, we advise you to make aliquots & store at -20°C. Once thawed, the Ligation buffer can be stored at 4°C.

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