CapeVARIII™ Reverse Transcription Kit

For cDNA synthesis and specialized applications

  • PCR, see protocol for first-strand cDNA synthesis
  • Second strand cDNA synthesis
  • DNA labelling
  • Reverse trancriptase quantitative PCR
  • Analysis of RNA by primer extension

The CapeVARIII™ Reverse transcriptase is a 75 kDa recombinant enzyme expressed and purified from a cloned Escherichia coli (E. coli) strain. This enzyme is a modified version of the wild-type MMLV with an enhanced cDNA synthesis performance. This enzyme is an RNA-dependent polymerase that catalyse the synthesis of RNA into cDNA. While perfectly suited for synthesizing cDNA from RNA, the enzyme cannot be used directly in targeted gene amplification PCR and must be coupled with CapeSprint™ Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications. 

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