CapeVARII™ Reverse Transcriptase Kit

For cDNA synthesis and specialized applications

PCR, see protocol for first-strand cDNA synthesis
Second strand cDNA synthesis
DNA labelling
Reverse trancriptase quantitative PCR
Analysis of RNA by primer extension

The CapeVARII™ Reverse transcriptase is a 75 kDa recombinant enzyme expressed and purified from a cloned Escherichia coli (E. coli) strain. This enzyme is a modified version of the wild-type MMLV with an enhanced cDNA synthesis performance.

This enzyme is an RNA-dependent polymerase that catalyse the synthesis of RNA into cDNA. While perfectly suited for synthesizing cDNA from RNA, the enzyme cannot be used directly in targeted gene amplification PCR and must be coupled with CapeSprint™ Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications.

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Product Specifications

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CapeVARII™ Reverse Transcription Kit
Contents	CapeVARII™ Reverse Transcription Enzyme, CapeVARII™ 10X RT Buffer, and Nuclease-free water
Cat. no	CV2E0050	CV2E0250	CV2E1000
No. of reactions	50	250	1000

	Specification
Applications	Reverse transcriptase PCR or cDNA synthesis
Real-time or endpoint	Both
dNTP’s included	No
Single or multiplex	Both
Hotstart	No