CapeVARII™ Reverse Transcriptase Kit
For cDNA synthesis and specialized applications
- PCR, see protocol for first-strand cDNA synthesis
- Second strand cDNA synthesis
- DNA labelling
- Reverse trancriptase quantitative PCR
- Analysis of RNA by primer extension
The CapeVARII™ Reverse transcriptase is a 75 kDa recombinant enzyme expressed and purified from a cloned Escherichia coli (E. coli) strain. This enzyme is a modified version of the wild-type MMLV with an enhanced cDNA synthesis performance.
This enzyme is an RNA-dependent polymerase that catalyse the synthesis of RNA into cDNA. While perfectly suited for synthesizing cDNA from RNA, the enzyme cannot be used directly in targeted gene amplification PCR and must be coupled with CapeSprint™ Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications.
CapeVARII™ Reverse Transcription Kit | |||
Contents | CapeVARII™ Reverse Transcription Enzyme, CapeVARII™ 10X RT Buffer, and Nuclease-free water | ||
Cat. no | CV2E0050 | CV2E0250 | CV2E1000 |
No. of reactions | 50 | 250 | 1000 |
Specification | |
Applications | Reverse transcriptase PCR or cDNA synthesis |
Real-time or endpoint | Both |
dNTP’s included | No |
Single or multiplex | Both |
Hotstart | No |
Please contact our sales professionals for this information.
cDNA Synthesis
- CapeVARI™ Reverse Transcription Kit
- CapeVARIII™ Reverse Transcription Kit
- CapeVARIV™ Reverse Transcription Kit
- Capebio™ One-Step Reverse Transcription qPCR Kit