Capebio™ T4 DNA Ligase (Enzyme Only)

Standard and specialized ligation experiments

  • Cloning of restriction enzyme generated fragments
  • QuickChange Mutation
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Cloning of PCR generated products
  • Self-circulation of linear DNA
  • AFLP
  • Ligation of double-stranded oligonucleotide linkers or adapters to DNA

Capebio™ T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, ligates DNA fragments with either cohesive or blunt termini. The enzyme requires ATP as a cofactor.

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