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HiFi Pfu DNA Polymerase ReadyMix is a ready-to-use mastermix, with Pfu DNA polymerase, dNTPs, PCR buffer, and stabilizers. The high-fidelity and strong 3’→5’ proofreading activity with a low error rate made the enzyme suited for its applications. The mastermix requires the addition of a DNA template, primers, and nuclease-free water. Designed to offer a convenient mastermix that minimises optimization and sample preparation time and reduces the risk of contamination.
Supplied concentration: 5X

Catalogue no	CHR0050	CHR0250	CHR1000
Number of reactions	50	250	1000
HiFi Pfu DNA Polymerase ReadyMix	250 µL	1250 µL	5000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 1 (A): Functionality assay.
(M) 1 Kb DNA marker.
(1) A 1.1 kb gene fragment amplified from plasmid DNA with HiFi Pfu polymerase.
(2)NTC control.
(B): Competitors assay.
(M)1 Kb DNA marker.
(1-2)HiFi Pfu (3-4) Competitor’s Pfu (5-6) NTC control.

Taq DNA Polymerase ReadyMix is a ready-to-use 5X reaction comprising Taq DNA Polymerase, dNTPs, PCR buffer and stabilizers. Simply add a DNA template, primers and nuclease-free water. Taq DNA Polymerase ReadyMix is a convenient mastermix format that minimises the need for optimisation and reduces pipetting steps, reduces the risk of contamination and increases throughput and reproducibility.
Supplied concentration: 5X

Catalogue no	CHR0050	CHR0250	CHR1000
Number of reactions	50	250	1000
5X Taq DNA Polymerase ReadyMix	313 µL	1563 µL	6252 µL
Nuclease-Free Water	1000 µL	1000 µL	1000 µL
Instruction for use	1	1	1

Figure 1: Functionality assay.
(M) 1 Kb DNA marker.
(1) PCR product of a 1.1 kb insert amplified from plasmid DNA with Taq polymerase.
(2) NTC control.

The Taq DNA Polymerase ReadyMix is a 5X ready-to-use mastermix designed to reduce PCR reaction setup, risk of contamination due to less handling and increased output. This product features a Taq DNA polymerase bound to an antibody to inhibit enzyme activity at temperatures below 50 °C which allows for sample preparation at room temperature (25 °C to 30 °C). This mastermix is formulated with dNTPs and buffer which contains enhancers and stabilisers to achieve the optimum product performance.
Supplied concentration: 5X

Catalogue no	CSR0050	CSR0250	CSR1000
Number of reactions	50	250	1000
Taq DNA Polymerase ReadyMix	250 µL	1250 µL	5000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 1: Functionality assay.
(M) 1 Kb DNA marker.
(2, 4) Gene fragment amplified from plasmid DNA with Taq DNA polymerase.
(3, 5) Negative control.

The HiFi DNA Polymerase ReadyMix is a 5X ready-to-use mastermix designed to reduce PCR reaction setup, risk of contamination due to less handling and increased output. This product features a hotstart HiFi Pfu DNA polymerase bound to an antibody to inhibit enzyme activity at temperatures below 50 °C which allows for sample preparation at room temperature (25 °C to 30 °C). This mastermix is formulated with dNTPs and buffer which contains enhancers and stabilizers to achieve the optimum product performance. The high-fidelity and strong 3’→5’ proofreading activity with a low error rate of Pfu DNA polymerase made this product well-suited for its applications. The mastermix only requires the addition of a DNA template, primers, and nuclease-free water.
Supplied concentration: 5X

Catalogue no	CTHR0050	CTHR0250	CTHR1000
Number of reactions	50	250	1000
5x HiFi Pfu DNA Polymerase ReadyMix	315 µL	1565 µL	6250 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 4: Functionality assay.
(M) 1 Kb DNA marker.
(1) Gene fragment amplified from plasmid DNA with HiFi Pfu DNA polymerase
(2) NTC control.

The Plus Taq DNA Polymerase ReadyMix is a 5X ready-to-use mastermix designed to reduce PCR reaction setup, risk of contamination due to less handling and increased output. This product features a hotstart Taq DNA polymerase and HiFi Pfu DNA polymerase bound to an antibody to inhibit enzyme activity at temperatures below 50 °C which allows for sample preparation at room temperature (25 °C to 30 °C). This mastermix is formulated with dNTPs and buffer which contains enhancers and stabilisers to achieve the optimum product performance. The high-fidelity and strong 3’→5’ proofreading activity with a low error rate of Pfu DNA polymerase and the high replicate rate of Taq DNA polymerase made this product well suited for its applications. The mastermix only requires the addition of a DNA template, primers, and nuclease-free water.
Supplied concentration: 5X

Catalogue no	CTR0050	CTR0250	CTR1000
Number of reactions	50	250	1000
5x Plus Taq DNA Polymerase ReadyMix	250 µL	1250 µL	5000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 5: Functionality assay.
(M) 1 Kb DNA marker.
(1) Gene fragment amplified from plasmid DNA with HiFi Pfu DNA polymerase
(2) NTC control.

Bst from Geobacillus stearothermophilus is a strand-displacement enzyme that enables DNA to be synthesized at a constant temperature, making it a powerful tool for isothermal amplification including Loop-Mediated Isothermal DNA Amplification (LAMP) that amplifies the target sequence using four or six primers. Bst exhibits a high level of displacement activity and 5’-3’ polymerase activity, however, it lacks 5’-3’ as well as 3’-5’ exonuclease activity. Bst DNA Polymerase 2X ReadyMix is a ready-to-use mastermix PCR application with any gene-specific primer sets and is suitable for both DNA (LAMP assay) and RNA (RT-LAMP assay) targets. It contains the Mutant Reverse Transcriptase, Cod Uracil-DNA Glycosylase Bst DNA Polymerase, dNTPs and buffer with enhancers.
Supplied concentration: 5X

Cod-Uracil DNA Glycosylase (UDG-Cod) enzyme catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in the uracil-containing single-stranded or double-stranded DNA and RNA. The enzyme can be added to a PCR premix to cleave off the uracil base (normally found in RNA) to prevent carryover of the base to the DNA strand during cDNA synthesis. UDG-Cod is ideal for use in conjunction with all PCR and RT-PCR enzymes, including DNA Polymerase and Reverse Transcriptase ranges.
Supplied concentration: 5X

HiFi Pfu SYBR Green qPCR Mix is a ready-to-use 2X mastermix designed for high-performance and reproducibility real-time PCR using SYBR Green. The Mix contains HiFi Pfu DNA polymerase, dNTPs, SYBR Green dye, buffer and stabilizers for qPCR using SYBR Green chemistry dye. Simply add DNA template, primers and nuclease-free water. The SYBR Green dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the number of dsDNA products generated during PCR. The Mix is compatible with many Real-time systems without requiring ROX reference dye.
Supplied concentration: 5X

Catalogue no	CHS0050	CHS0250	CHS1000
Number of reactions	50	250	1000
5x HiFi Pfu SYBR Green qPCR Mix	500 µL	2500 µL	10000 µL
Nuclease-Free Water	1000 µL	1000 µL	1000 µL
Instruction for use	1	1	1

Figure 8: Amplification curve for SARS-COV-2 S gene with HiFi Pfu SYBR Green qPCR Mix.

The MMLV Reverse Transcriptase is a wild-type Moloney Murine Leukemia Virus (MMLV). It is a 75 kDa recombinant enzyme expressed and purified from Escherichia coli (E. coli) strain with an enhanced cDNA synthesis performance. This enzyme is an RNA-dependent polymerase that catalyses the synthesis of RNA into cDNA. Suited for synthesizing cDNA from RNA, the enzyme cannot be used directly for PCR-targeted gene amplification and must be coupled with Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) can be used in cDNA synthesis with long messenger RNA templates (>5kb). The MMLV reverse transcriptase enzyme is a 5X a ready-to-use mastermix that contains MMLV reverse transcriptase enzyme, dNTPs, and buffer with enhancers.
Supplied concentration: 5X

Catalogue no	CME0100	CME0250	CME1000
Number of reactions	100	250	1000
MMLV Reverse Transcriptase Enzyme ReadyMix	400 µL	1000 µL	4000 µL
Nuclease-Free Water	500 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 9: Reliable detection of SARS-CoV-2 with MMLV Reverse Transcriptase Enzyme ReadyMix in a One-Step RT-qPCR on QuantStudio™ 5 Real-Time PCR System.

One-Step Multiplex 4X ReadyMix is a ready-to-use mastermix for real-time PCR applications with any gene-specific primer and probe sets and is suitable for both DNA and RNA targets. It contains the Mutant Reverse Transcriptase, Cod Uracil-DNA Glycosylase hot-start Taq DNA Polymerase, dNTPs and buffer with enhancers. Optimized buffer components allow for multiplex amplification of up to four DNA or RNA target sequences in a single reaction. This kit has been proven to provide efficient, sensitive, and specific results.
Supplied concentration: 4X

Catalogue no	COM02001	COM02001
Number of reactions	100	500
One-Step Multiplex 4X ReadyMix	1000 µL	2500 µL
Nuclease-Free Water	2000 µL	2000 µL
Instruction for use	1	1

Figure 10: Reliable detection of SARS-CoV-2 with One-Step Multiplex ReadyMix.

One-Step RT-PCR ReadyMix is a ready-to-use mastermix. It contains the Mutant Reverse Transcriptase, Cod Uracil-DNA Glycosylase, Taq DNA polymerase enzymes and buffer with enhancers that are optimized for the convenient generation of complimentary DNA and its subsequent amplification in a single tube. The ReadyMix can be utilized for the in vitro detection of RNA extracted from human clinical samples and environmental samples using gene-specific primer and probe sets. This ReadyMix has been proven to provide efficient, sensitive, and specific results.
Supplied concentration: 4X

Catalogue no	CORT05001	CORT10001
Number of reactions	500	1000
One-Step Reverse Transcription qPCR ReadyMix	1000 µL	2500 µL
Nuclease-Free Water	2000 µL	2000 µL
Instruction for use	1	1

Figure 11: Reliable detection of SARS-CoV-2 with One-Step RT-qPCR ReadyMix.

Proteinase K enzyme produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage specificity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Useful for general digestion of protein in biological samples. The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications, including the preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting, and removal of nucleases from preparations of DNA and RNA.
Supplied concentration: 20 mg/mL

Catalogue no	CPK0050	CPK0250	CPK1000
Number of reactions	50	250	1000
Proteinase K Enzyme	125 µL	625 µL	2500 µL
Nuclease-Free Water	1000 µL	1000 µL	1000 µL

Figure 1: Activity assay with 1mg/mL of a competitor and Proteinase K on 16 mg/mL BSA at 37 °C.
1: BSA,
2: Competitor Proteinase K after 15 min,
3: Competitor proteinase K after 45 min,
4: Negative control,
5: Proteinase K after 15 min,
6: Proteinase K after 45 min.
Proteinase K is also able to completely digest 16 mg/mL of BSA after 15 min.

The T4 DNA Ligation ReadyMix is a ready to use mastermix that catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. This enzyme will repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. The enzyme requires ATP as a cofactor. This product has proven to be a highly stable enzyme that exhibits activity even after long-term storage and is designed to reduce sample preparation steps.
Supplied concentration: 5X

The VARII Reverse Transcriptase is a 75 kDa recombinant enzyme produced in Escherichia coli. This enzyme is a modified version of the wild-type Moloney Murine Leukemia Virus (MMLV). Designed to offer enhanced cDNA synthesis performance and RNA dependent polymerase activity that catalyses the synthesis of RNA into cDNA. The enzyme cannot be used directly for PCR-targeted gene amplification and must be coupled with Taq DNA Polymerase for reverse transcription PCR (RT-PCR) applications. The VARII Reverse Transcriptase enzyme is a 5X ready-to-use mastermix that contains MMLV reverse transcriptase enzyme, dNTPs, and buffer with enhancers.
Supplied concentration: 20 mg/mL

Catalogue no	CVE0100	CVE0250	CVE1000
Number of reactions	100	250	1000
VARII Reverse Transcriptase Enzyme	100 µL	250 µL	500 µL
Nuclease-Free Water	500 µL	1000 µL	2000 µL
Nuclease-Free Water	1	1	1

Figure 13: Reliable detection of SARS-CoV-2 with VARII Reverse Transcriptase Enzyme ReadyMix in a One-Step RT-qPCR on QuantStudio™ 5 Real-Time PCR System.

Taq SYBR Green qPCR Mix® is a ready-to-use 2X mastermix designed for high-performance and reproducibility real-time PCR using SYBR Green. The kit contains engineered Taq DNA polymerase, dNTPs, SYBR Green dye, buffer and stabilizers for qPCR using SYBR Green chemistry dye. Simply add DNA template, primers and nuclease-free water. The SYBR Green dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the number of dsDNA products generated during PCR. The kit is compatible with many Real-time systems without requiring ROX reference dye.
Supplied concentration: 2X

Catalogue no	CSS0050	CSS0250	CSS1000
Number of reactions	100	250	1000
Taq SYBR Green qPCR Mix	50 µL	2500 µL	10000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Nuclease-Free Water	1	1	1

Figure 14: Amplification curve for SARS-COV-2 S gene with Taq SYBR Green qPCR Mix.

DNase I (deoxyribonuclease) is a recombinant 29 kDa, a highly thermostable enzyme isolated from Bovine and expressed in E. coli. DNase I is an endonuclease that digests single- or double-stranded DNA by cleaving the phosphodiester bonds between the nucleotides. The enzyme hydrolyses phosphodiester bonds to produce mono- and oligodeoxynucleotides containing 5’- phosphate group and 3′-hydroxy group. The buffer co-factors optimize the enzyme conformation and are involved in the cleavage of the DNA phosphodiester bonds.
Supplied concentration: 1 mg/mL

Catalogue no	CDIK0250	CDIK0500	CDIK1000
Number of reactions	250	500	1000
DNase I Enzyme	500 µL	1000 µL	2000 µL
Nuclease-Free Water	1000 µL	1000 µL	1000 µL
Nuclease-Free Water	1	1	1

Figure 15: Functionality assay. (1) Undigested Plasmid DNA, (2) DNase I treatment for 5 mins (3) DNase I treatment for 30 mins.

RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA. RNases function by enzymatically breaking down RNA and are essential for many research applications. Digestion of RNA is often required, for example, when purifying proteins and DNA. Recombinantly, purified from E. coli.
Supplied concentration: 5 mg/mL

Catalogue no	CRA0050	CRA0250	CRA1000
Number of reactions	50	250	1000
DNase I Enzyme	100 µL	500 µL	2000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 17: Functionality and Competitor’s assays.
(1) Negative control,
(2) RNase A treatment for 30 mins (Capebio)
(3) RNase A treatment for 30 mins (Competitor)
(4) Negative control

Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades single-stranded, double-stranded, linear, and circular DNA and RNA forms without proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. It completely digests nucleic acids to 5’-monophosphate terminated oligonucleotides 3 to 5 bases in length (below the hybridization limit), which is ideal for the removal of nucleic acids from recombinant proteins enabling compliance with FDA guidelines for nucleic acid contamination.
Supplied concentration: 10 mg/mL

Catalogue no	CBN0250	CBN0500	CBN1000
Number of reactions	250	50	1000
Benzonase Nuclease	250 µL	500 µL	1000 µL
Nuclease-Free Water	1000 µL	1000 µL	2000 µL
Instruction for use	1	1	1

Figure 8: Functionality assay.
(M) 1 Kb DNA marker, Lane:
(1) Lambda
(2) NTC
(3) Benzonase nuclease treatment after 30 mins.